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KMID : 0380620130450060777
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Korean Journal of Food Science and Technology
2013 Volume.45 No. 6 p.777 ~ p.784
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Isolation and Identification of Antioxidants from Methanol Extract of Sword Bean (Canavalia gladiata)
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Kim Jong-Pil
Lee Hyang-Hee
Moon Jae-Hak
Ha Dong-Ryong
Kim Eun-Sun
Kim Jin-Hwan
Seo Kye-Won
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Abstract
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The ethyl acetate (EtOAc) layer of Canavalia gladiata (sword bean) methanol extracts showed higher 1,1- diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity than other layers. Four phenolic compounds were isolated from the EtOAc layer by silica gel column chromatography and prep-HPLC using a guided DPPH radical-scavenging assay. The isolated compounds were identified as methyl gallate (1), gallic acid (2), 1,6-di-O-galloyl β-D-glucopyranoside (3), and 1,4,6-tri-O-galloyl β-D-glucopyranoside (4) based on MS and NMR analyses. Among the four compounds, no. 4 was isolated from this plant for the first time. Their DPPH radical-scavenging activities based on SC50 decreased in the following order: 4 (6.9 μM)>3 (8.3 μM)>2 (10.0 μM)>1 (10.3 μM).
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KEYWORD
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Canavalia gladiata, sword bean, 1, 4, 6-tri-O-galloyl β-D-glucopyranoside, galloyl glucoside, gallic acid derivative
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